ELISA Development

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Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used assay in diagnostics, drug discovery, clinical development, and other life-science industries. This is a sensitive assay that detects and quantifies a target molecule in biological fluids or solutions. ELISA can be performed in various formats and platforms. Although a target-specific antibody is the central aspect of an ELISA, other components are also critical for assay performance. Our seamless, integrated expertise in antibody-antigen biochemistry, functional assays, recombinant protein production, hybridoma cell culture, antibody purification, characterization, and conjugation allows us to deliver a fully optimized and validated ELISA for its intended final application.

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ELISA TYPES

• direct

• indirect

• sandwich

• competition

• inhibition

ELISA DETECTION OPTIONS

• visible absorbance

• fluorescence

• luminescence

ELISA DESIGN INCLUDES A SELECTION OF:

• assay format

• antibody or antibody pair

• standards

• palate-coating chemistry

• blocking and wash buffers

• detection strategy

• detection reagents

ELISA OPTIMIZATION INVOLVES TESTING OF VARIOUS:

• concentrations of antibodies, samples, and buffers

• coating protocols

• detection protocols

• chessboard titrations

ELISA VALIDATION PARAMETERS ARE TESTED:

• robustness / precision / trueness

• uncertainty

• limit of quantification

• dilutional linearity

• parallelism

• recovery

• selectivity

• sample stability

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       Got Questions?  E-mail: support@atcs-incorporated.com

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