ELISA Development

Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used assay in diagnostics, drug discovery, clinical development, and other life-science industries. This is a sensitive assay that detects and quantifies a target molecule in biological fluids or solutions. ELISA can be performed in various formats and platforms. Although a target-specific antibody is the central aspect of an ELISA, other components are also critical for assay performance. Our seamless, integrated expertise in antibody-antigen biochemistry, functional assays, recombinant protein production, hybridoma cell culture, antibody purification, characterization, and conjugation allows us to deliver a fully optimized and validated ELISA for its intended final application.

ELISA TYPES

  • direct

  • indirect

  • sandwich

  • competition

  • inhibition


ELISA DETECTION OPTIONS

  • visible absorbance

  • fluorescence

  • luminescence


ELISA DESIGN INCLUDES A SELECTION OF:

  • assay format

  • antibody or antibody pair

  • standards

  • palate-coating chemistry

  • blocking and wash buffers

  • detection strategy

  • detection reagents


ELISA OPTIMIZATION INVOLVES TESTING OF VARIOUS:

  • concentrations of antibodies, samples, and buffers

  • coating protocols

  • detection protocols

  • chessboard titrations


ELISA VALIDATION PARAMETERS ARE TESTED:

  • robustness / precision / trueness

  • uncertainty

  • limit of quantification

  • dilutional linearity

  • parallelism

  • recovery

  • selectivity

  • sample stability

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